One of Seqomics’ missions is to enhance research collaborations, contracting between BayGen Institute and potential domestic and international partners.

1. Resequencing, Deep sequencing

• whole microbial genomes, BAC clones, SNP analyses

The main goal of resequencing projects is basically to identify SNPs and other polymorphisms, such as insertions and deletions (called indels). SNP discovery is essential for genetic mapping in eukaryotic organisms having large, complex genomes.

2. Metagenomics

Dynamic changes, metabolic activities of microbial populations can be monitored using next generation sequencing. Most microbial species are not culturable, and even within a species there can be huge variations in genotype (and consequently phenotype) owing to genetic plasticity.

3. RNA-Seq

RNA-Seq is a serious competitor of traditional whole transcriptome analysis methods (microarray based methods). Next Generation Sequencing made  possible to perform whole transcriptome analysis in digital way (digital gene expression analysis). SOLiD method can be used quantitatively over five orders of magnitude and is not dependent on previous knowledge of transcribed sequences.

4. ChIP-Seq

Chromatin immunoprecipitation using microarrays (ChIP-on-chip) is a high-throughput method for discovering protein-binding sites in DNA on a whole-genome scale. It is now possible to directly determine the bound DNA sequence using next-generation sequencing technologies with all the usual advantages of digital methods. This approach, named ChIP–Seq, has already been used to discover transcription factor binding sites in humans and in microorganisms.

5. Micro-RNA discovery

The availability of high-throughput sequencing has led to the discovery of thousands of new species of non-coding sRNAs, especially in plants. The short read length of these sequencing platforms is not a limitation for sRNA discovery.

Last Updated ( Thursday, 10 September 2009 14:06 )